abstract：:Three isoenzymes of UDP-glucuronyltransferase (UDPGT) have been separated and purified from liver microsomes of untreated female rats or female rats pretreated with 3-methylcholanthrene. The UDPGT isoenzymes were purified utilizing Chromatofocusing, column isoelectric focusing, and UDP-hexanolamine Sepharose 4B affini...

journal_title：Archives of biochemistry and biophysics

authors： Falany CN,Tephly TR

更新日期：1983-11-01 00:00:00

abstract：:Organic hydroperoxides can replace NADPH in supporting the oxidation of ethanol by liver microsomes. Experiments were carried out to evaluate the role of hydroxyl radicals in the organic hydroperoxide-catalyzed reaction. Maximum rates of ethanol oxidation occurred in the presence of either 0.5 mM cumene hydroperoxide ...

journal_title：Archives of biochemistry and biophysics

authors： Cederbaum AI

更新日期：1983-11-01 00:00:00

abstract：:Using Escherichia coli guanosine triphosphate cyclohydrolase, dihydroneopterin triphosphate was synthesized from guanosine triphosphate and was compared with sepiapterin as a substrate for tetrahydrobiopterin formation in bovine adrenal medulla extracts. The dihydrofolate reductase inhibitor, methotrexate, blocks the ...

journal_title：Archives of biochemistry and biophysics

authors： Smith GK,Nichol CA

更新日期：1983-11-01 00:00:00

abstract：:As a means of comparing the functional properties of an enzyme in dilute solution in vitro with those for the same enzyme acting in its normal cellular environment, a study was conducted with 4-substituted pyrazoles as inhibitors of rat liver alcohol dehydrogenase in vitro and ethanol oxidation in isolated rat hepatoc...

journal_title：Archives of biochemistry and biophysics

authors： Cornell NW,Hansch C,Kim KH,Henegar K

更新日期：1983-11-01 00:00:00

abstract：:The glutamylation of methotrexate has been evaluated in H35 hepatoma cells in vitro as a function of the conditions of culture. Glutamylation yields methotrexate polyglutamate with two to five additional glutamate residues and is a saturable process. The rate of glutamylation increases little above 10 microM extracell...

journal_title：Archives of biochemistry and biophysics

authors： Nimec Z,Galivan J

更新日期：1983-10-15 00:00:00

abstract：:Uteroglobin has been purified from hare lung by gel filtration and chromatography on carboxymethyl-cellulose. Hare uteroglobin appears homogeneous by electrophoresis under both denaturing and nondenaturing conditions. Its chemical and immunological properties as well as its ability to bind progesterone are compared to...

journal_title：Archives of biochemistry and biophysics

authors： Soledad López de Haro M,Nieto A

更新日期：1983-10-15 00:00:00

abstract：:Glucocorticoids will enhance the growth of cultured human skin fibroblasts in serum-containing medium. In serum-free cultures hydrocortisone (5 X 10(-6) M) will enhance insulin stimulation of sugar transport and DNA synthesis (as measured by thymidine incorporation into trichloroacetic acid-precipitable material). The...

journal_title：Archives of biochemistry and biophysics

authors： Germinario RJ,McQuillan A,Oliveira M,Manuel S

更新日期：1983-10-15 00:00:00

abstract：:Factors influencing pyrroline 5-carboxylate (P5C) synthesis from glutamate by a subcellular fraction enriched in mitochondria of rat small intestinal mucosa have been studied. P5C synthesis decreased rapidly if this subcellular fraction was preincubated at 20 degrees C in the absence of substrates; this effect suggest...

journal_title：Archives of biochemistry and biophysics

authors： Henslee JG,Wakabayashi Y,Small C,Jones ME

更新日期：1983-10-15 00:00:00

abstract：:Fructose 2,6-bisphosphate inhibited all three fructose-1,6-bisphosphatases from the liver, intestine, and muscle of the mouse. The sensitivity of the liver enzyme to the inhibitor was significantly diminished when Mg2+ was replaced by Mn2+ as the activating cation. Inhibition of the liver enzyme by fructose 2,6-bispho...

journal_title：Archives of biochemistry and biophysics

authors： Mizunuma H,Tashima Y

更新日期：1983-10-01 00:00:00

abstract：:The first evidence for the reductive metabolism of a noncyclic nitrosamine to the corresponding hydrazine derivative in vivo and in vitro is provided. Under anaerobic conditions, N-nitrosodiphenylamine was reduced by guinea pig liver 9000g supernatant to 1,1-diphenylhydrazine in the presence of 2-hydroxypyrimidine or ...

journal_title：Archives of biochemistry and biophysics

authors： Tatsumi K,Yamada H,Kitamura S

更新日期：1983-10-01 00:00:00

abstract：:The acute effect of ethanol on hepatic protein synthesis is a rather controversial issue. In view of the conflicting reports on this subject, the effect of ethanol on protein labeling from L-[3H]valine in isolated liver cells was studied under a variety of experimental conditions. When tracer doses of the isotope were...

journal_title：Archives of biochemistry and biophysics

authors： Girbes T,Susin A,Ayuso MS,Parrilla R

更新日期：1983-10-01 00:00:00

abstract：:The reaction center of photosystem 2 has been highly purified from digitonin-solubilized thylakoid membranes of the thermophilic cyanobacterium Synechococcus sp. by means of sucrose density gradient centrifugation and electrophoresis on polyacrylamide gels containing digitonin. Sodium dodecyl sulfate-polyacrylamide ge...

journal_title：Archives of biochemistry and biophysics

authors： Yamagishi A,Katoh S

更新日期：1983-09-01 00:00:00

abstract：:Periodate treatments of apo human serum transferrin (HST), and apo chicken ovotransferrin (COT) were previously reported to cause a rapid loss of Fe+3 binding capacity, with a loss of 3 to 5 tyrosine residues [P. AZARI AND J. L. PHILLIPS (1970) Arch. Biochem. Biophys. 138, 32-38; K. F. GEOGHEGAN, J. L. DALLAS, AND R. ...

journal_title：Archives of biochemistry and biophysics

authors： Penner MH,Yamasaki RB,Osuga DT,Babin DR,Meares CF,Feeney RE

更新日期：1983-09-01 00:00:00

abstract：:A chromatographic adsorbent (TED-Sepharose) capable of chelating various di- and trivalent metal ions was prepared by introducing tris(carboxymethyl)ethylenediamine into agarose. This gel was charged with Al3+, Ga3+, In3+, and Tl3+ to form immobilized-metal affinity adsorbents, and their adsorption behavior toward ser...

journal_title：Archives of biochemistry and biophysics

authors： Porath J,Olin B,Granstrand B

更新日期：1983-09-01 00:00:00

abstract：:The binding site for ATP to tubulin was established by use of the photoaffinity label [gamma-32P]N3ATP. Photolysis of the analog in the presence of tubulin resulted in covalent modification of the protein as revealed by autoradiography of electropherograms. Scanning the autoradiograms showed that the ATP analog was bo...

journal_title：Archives of biochemistry and biophysics

authors： Zabrecky JR,Cole RD

更新日期：1983-09-01 00:00:00

abstract：:The isolation and purification of a 21,000-Da (pI 4.9) novel protein from porcine anterior pituitary and whole human pituitary is described. Comparison of the NH2-terminal sequence of the first 77 and 81 residues of the human and porcine homologs shows only one conservative substitution at residue 12, namely an Ala fo...

journal_title：Archives of biochemistry and biophysics

authors： Seidah NG,Hsi KL,De Serres G,Rochemont J,Hamelin J,Antakly T,Cantin M,Chrétien M

更新日期：1983-09-01 00:00:00

abstract：:The major proteins to which 3-methylcholanthrene specifically binds have been purified over 480-fold with a 45% yield compared to a rat liver 100,000g supernate. The procedure involved a batch ion-exchange technique together with hydrophobic gel filtration and chromatofocusing chromatography. The multiple, specific 3-...

journal_title：Archives of biochemistry and biophysics

authors： Tierney B,Munzer S,Bresnick E

更新日期：1983-09-01 00:00:00

abstract：:Tritium-labeled 24(R)-hydroxyvitamin D3 and 24(S)-hydroxyvitamin D3 were chemically synthesized and the 1 alpha-hydroxylation of these compounds by chick kidney homogenates was studied. A marked stereospecific preference with regard to the orientation of the hydroxyl functionality on carbon-24 was noted: while the 24(...

journal_title：Archives of biochemistry and biophysics

authors： Ishizuka S,Bannai K,Norman AW

更新日期：1983-09-01 00:00:00

abstract：:In order to search for probable conformations of the peptide, the amino acid side chain, and the carbohydrate linkage in glycoproteins, conformational energy surfaces of glycopeptide model compounds were studied by Monte Carlo methods using the Metropolis algorithm. The potential energies were composed of empirical en...

journal_title：Archives of biochemistry and biophysics

authors： Duben AJ,Bush CA

更新日期：1983-08-01 00:00:00

abstract：:A quantitative assay has been developed to measure holocarboxylase synthetase activity in cellular extracts. This assay was based on measuring the incorporation of [3H]biotin of high specific activity (4.3 Ci/mmol) into purified rat liver apopyruvate carboxylase. With this assay, holocarboxylase synthetase in 3T3-L1 m...

journal_title：Archives of biochemistry and biophysics

authors： Chang HI,Cohen ND

更新日期：1983-08-01 00:00:00

abstract：:The short term response of the L-6 cell line of rat skeletal myoblasts to elevated extracellular iron concentrations was studied. It was found in all cases that iron as the nitrilotriacetate (NTA) chelate was effective at donating iron to the cells and at stimulating ferritin synthesis. After 48 h in 50 microM ferric ...

journal_title：Archives of biochemistry and biophysics

authors： Rittling SR,Woodworth RC

更新日期：1983-08-01 00:00:00

abstract：:The ability of nine phospholipids to alter the activity of low-Km cyclic AMP phosphodiesterase was examined in microsomal fractions of rat adipocytes. The enzyme was activated by phosphatidylserine (21% at 300 microM) and phosphatidylglycerol (36% at 300 microM). The activation was concentration dependent over the ran...

journal_title：Archives of biochemistry and biophysics

authors： Macaulay SL,Kiechle FL,Jarett L

更新日期：1983-08-01 00:00:00

abstract：:This study systematically examined the characteristics of specific binding of adult diferric transferrin to its receptor using a Triton X-100 solubilized preparation from human placentas as the receptor source. The following information was obtained. The ionic strength for maximal binding is in the range of 0.1-0.3 M ...

journal_title：Archives of biochemistry and biophysics

authors： Tsunoo H,Sussman HH

更新日期：1983-08-01 00:00:00

abstract：:A series of esters of adenosine 5'-monophosphate with ethyl, propyl, or hexyl moieties substituted at the omega-position with chlorine or bromine were prepared. The compounds were competitive inhibitors of horse liver alcohol dehydrogenase with respect to coenzyme, NAD+, and had inhibition (dissociation) constants in ...

journal_title：Archives of biochemistry and biophysics

authors： Fries RW,Bohlken DP,Murch BP,Leidal KG,Plapp BV

更新日期：1983-08-01 00:00:00

abstract：:By exploiting its capacity for binding to DNA, the protease inhibitor alpha 1-antichymotrypsin has been isolated from human serum by ammonium sulfate fractionation and successive chromatography on QAE-Sephadex, DNA-cellulose, and Sephacryl S-300. This experimental procedure compares favorably with existing methods for...

journal_title：Archives of biochemistry and biophysics

authors： Abdullah M,Siddiqui AA,Hill JA,Davies RJ

更新日期：1983-08-01 00:00:00

abstract：:The photoreduction of soluble plastocyanin in a homologous reconstituted system requires a resolved Photosystem II preparation and the cytochrome b6-f complex from spinach chloroplasts. Oxygen evolution is linked quantitatively to plastocyanin reduction in the reconstituted system, indicating that water is the electro...

journal_title：Archives of biochemistry and biophysics

authors： Lam E,Malkin R

更新日期：1983-07-15 00:00:00

abstract：:The time-course kinetics of the cytochrome P-450-catalyzed dealkylations of the exogenous compounds benzphetamine, ethylmorphine, codeine, and 7-ethoxycoumarin were compared to the hydroxylation of the endogenous compound testosterone. Using liver microsomes from phenobarbital-induced rats, the time course of the deme...

journal_title：Archives of biochemistry and biophysics

authors： Hamill SJ,Cooper DY,Schleyer H,Rosenthal O

更新日期：1983-07-15 00:00:00

abstract：:Swainsonine, a toxic plant alkaloid reported to be the agent that induces in animals a neurological condition very similar to the hereditary lysosomal storage disease mannosidosis, and to inhibit the formation of complex glycoproteins of the asparagine-linked class, was recently shown [D.R.P. Tulsiani, T.M. Harris, an...

journal_title：Archives of biochemistry and biophysics

authors： Tulsiani DR,Touster O

更新日期：1983-07-15 00:00:00

abstract：:Inducible formation of ribulose-1,5-bisphosphate (RuBP) carboxylase in the cells of Chromatium vinosum under autotrophic conditions was not affected by six different inhibitors of DNA synthesis. Photosynthetic CO2 fixation and RuBP carboxylase activities were not influenced by seven reagents known to eliminate plasmid...

journal_title：Archives of biochemistry and biophysics

authors： Kobayashi H,Akazawa T

更新日期：1983-07-01 00:00:00

abstract：:A 60- to 70-fold purification of an NAD+ glycohydrolase from the inner membrane of rat liver mitochondria to apparent homogeneity on sodium dodecyl sulfate (SDS)-polyacrylamide slab gel is described. The minimum molecular weight of the enzyme on polyacrylamide gels in the presence of SDS is around 62,000. The enzyme s...

journal_title：Archives of biochemistry and biophysics

authors： Moser B,Winterhalter KH,Richter C

更新日期：1983-07-01 00:00:00

abstract：:The susceptibility of 40S and 60S ribosomal subunits from Saccharomyces cerevisiae to digestion with varying concentrations of trypsin was studied by two-dimensional electrophoresis and quantitative measurements of the protein remaining with the ribosomal particles after trypsin treatment. Proteins from both subunits ...

journal_title：Archives of biochemistry and biophysics

authors： Lee JC,Haley WT Jr

更新日期：1983-07-01 00:00:00

abstract：:Prostaglandin E (PGE) stimulates resorption in bone. Since osteoblast-like osteosarcoma cells secrete PGE2, the possibility that osteoclasts were the major target for PGE was considered. To study this question, it was first established that in isolated bone cells enriched for either osteoclastic (OC) or osteoblastic (...

journal_title：Archives of biochemistry and biophysics

authors： Wong GL,Kocour BA

更新日期：1983-07-01 00:00:00

abstract：:Immunological cross-reactivities between isolated subunits of the scorpion Androctonus australis (Aa) and of the horseshoe crab Limulus polyphemus (Lp) hemocyanins were studied using subunit-specific antibodies prepared through immunoadsorption to pure immobilized subunits. Rocket immunoelectrophoreses of the various ...

journal_title：Archives of biochemistry and biophysics

authors： Lamy J,Compin S,Lamy JN

更新日期：1983-06-01 00:00:00

abstract：:The relationship between release of membrane lipid peroxidation products and phospholipase action was examined. Rat liver microsomes and phosphatidylcholine liposome-phospholipase A2 preparations were subjected to iron ascorbate-induced lipid peroxidation. Peroxidation products were characterized by measurement of mal...

journal_title：Archives of biochemistry and biophysics

authors： Sevanian A,Muakkassah-Kelly SF,Montestruque S

更新日期：1983-06-01 00:00:00

abstract：:The binding of the ligands Mn2+, Ca2+, and methyl alpha-D-glucopyranoside to concanavalin A, purified as described (A.J. Sophianopoulos and J.A. Sophianopoulos (1981) Prep. Biochem. 11, 413-435), was studied by ultrafiltration in 0.2 M NaCl, pH 5.2 and pH 6.5 to 7, and at 23 to 25 degrees C. The association constant (...

journal_title：Archives of biochemistry and biophysics

authors： Sophianopoulos JA,Sophianopoulos AJ,MacMahon WC

更新日期：1983-06-01 00:00:00

abstract：:The stability of glutathione peroxidase was assessed in vitro via oxidative inactivation by peroxides and a peroxidizing fatty acid and by renaturation and proteolysis. The stability of glutathione peroxidase to methyl ethyl ketone peroxide, H2O2, linoleic acid hydroperoxide, and peroxidizing methyl linolenate was com...

journal_title：Archives of biochemistry and biophysics

authors： Condell RA,Tappel AL

更新日期：1983-06-01 00:00:00

abstract：:Valproic acid (dipropylacetic acid), an antiepileptic agent known to be hepatotoxic in some patients, caused inhibition of lactate gluconeogenesis, fatty acid oxidation, and fatty acid synthesis by isolated hepatocytes. The latter process was the most sensitive to valproic acid, 50% inhibition occurring at ca. 125 mic...

journal_title：Archives of biochemistry and biophysics

authors： Becker CM,Harris RA

更新日期：1983-06-01 00:00:00

abstract：:Ochratoxin A, a nephrotoxin produced by Aspergillus ochraceus, decreases the activity of phosphoenolpyruvate carboxykinase (GTP) (EC 4.1.1.32) (PEPCK) in the cytosol of rat kidneys, as well as inhibits renal gluconeogenesis (Meisner, H., and Meisner, P. (1981) Arch. Biochem. Biophys. 208, 146-153). Ochratoxin A greatl...

journal_title：Archives of biochemistry and biophysics

authors： Meisner H,Cimbala MA,Hanson RW

更新日期：1983-05-01 00:00:00

abstract：:Xanthine dehydrogenase was purified from soybean nodules and the kinetic properties were studied at pH 7.5. Km values of 5.0 +/- 0.6 and 12.5 +/- 2.5 microM were obtained for xanthine and NAD+, respectively. The pattern of substrate dependence suggested a Ping-Pong mechanism. Reaction with hypoxanthine gave Km's of 52...

journal_title：Archives of biochemistry and biophysics

authors： Boland MJ,Blevins DG,Randall DD

更新日期：1983-04-15 00:00:00

abstract：:Cellular distribution in rat livers of the mannan-binding protein, a liver lectin specific for mannose and N-acetylglucosamine was investigated. Estimation of mannan binding activity of isolated cells demonstrated that more than 99% of the mannan binding activity recovered from whole livers was derived from hepatocyte...

journal_title：Archives of biochemistry and biophysics

authors： Mori K,Kawasaki T,Yamashina I

更新日期：1983-04-15 00:00:00